Download Animal Cell Technology. Developments, Processes and Products by R. E. Spier PDF

By R. E. Spier

ISBN-10: 0750604212

ISBN-13: 9780750604215

This e-book offers a cutting-edge record at the box of animal mobile expertise, a resource of reference for all these enthusiastic about the construction and use of animal mobile items

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2. 3 or rmAB-3. No evidence of instability of specific productivity was seen in extended flask culture for either of these cell lines, whether cultured in the presence or absence of MSX (figures 3 & 4). The gs- NSO system has advantages over gs- CHO cell lines. lOOuM). This may contribute to a more inherently stable genotype than the high copy number gs-CHO system. Furthermore the NSO host cell line lacks an endogenous gs gene, and so glutamine- free growth of producer gs-NSO cell lines without MSX addition will still maintain at least partial selection pressure.

Cell Biol. Int. Rep. 1980, 4, 149-153. 28 IMMORTALISATION OF FLATFISH (PARALICHTHYS OLIVACEUS) LEUKOCYTES BY ONCOGENE TRANSFECTION Tadakazu Tamai*, Nobuyuki Sato*, Shoji Kimura*, Sanetaka Shirahata+ and Hiroki Murakami* *Taiyo Central R&D Institute, Taiyo Fishery Co. , 16-2 Wadai, Tsukuba-shi, Ibaraki 300-42, Japan +Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812, Japan ABSTRACT Flatfish leukocytes were transfected with the plasmids expressing the v-myc, c-myc, c-fos, v-myb and c-Ha-ras oncogenes under the control of CMV or SV40 early promoter.

The levels of mRNA of oncogene in the transfected cells were as low as the level of most kinds of mRNA in cells. Higher level of expression of oncogenes might be necessary to obtain stable highly productive cells for exogenous genes by activation of specific promoter in transfected cells. To produce more stable and higher productive cells by the use of on­ cogenes, higher level of expression of oncogenes, development of more sensitive promoter for oncogene activation, insertion of more copy num­ ber of oncogenes and exogenous genes into chromosomes will be necessary.

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